1084 replies to this topic

[niner]

I'm just a little surprised that the same people who complained about the effects of emodin are now using 99% resveratrol and mixing it with a laxative. But if it makes them run faster ...

That was my thought, Lucid or any others, any laxitive effects from the PEG aka Miralax?

I've been mixing my 99% t-res with lecithin and olive oil and taking it along with various polyphenols including quercetin and yes bioperine (ive been converted). I'm a little afraid of anything called Miralax

I've been playing around with the proportion of Miralax to resveratrol recently. I've increased the dose of Miralax up to about 3 grams, based on my observation that I can get a small quantity of resveratrol to appear to dissolve in an aqueous solution of Miralax. My interpretation is that I'm forming a soluble complex between the two compounds, but with my earlier dose of about a gram of Miralax, this complex-forming ability was quickly saturated. The ability of the PEG to solubilize resveratrol appears to be roughly linear in PEG concentration, on the order of roughly 50-100mg resveratrol per gram of Miralax. At one gram I had no problem with laxative effects. At three grams every other day, I'm noticing looser stools, but nothing that's a problem. Miralax is a pretty gentle laxative. All it does is cause the stool to hold more water. You'd probably get a similar effect if you just drank a lot more liquid. (Which is a pretty good laxative itself, imho).

[niner]

Really, I think we should have some rigorous 'Albert' tests for many of the methods listed in this thread to check bioavailability. As a matter of fact, it should be industry standard to Albert test all resv supps and administration methods. Has anyone contact David Sinclair yet about giving SIRT501 the Albert test yet?

The 'Albert' test, or any of the other inflammation-based tests we use, may be the best we have, but they are far from rigorous. The right way to evaluate bioavailability is to draw samples of blood at a series of times after dosing, and analyze them for free resveratrol. Anthony is looking into this now; if he can manage it, we'll be able to get a lot of this on a much firmer footing.

(Go Anthony!)

[missminni]

I have no idea if this is from resveratrol or something else,
but I broke out in massive hives on my right thigh this evening. They don't itch but
they look terrible. They covered the whole front and outside of my thigh.
I haven't introduced any new supplement, and I didn't change the amount of res, so
I am wondering can the reaction be from a cumulative effect of res?maybe dmso?
And why my thigh? I've never applied it there. I won't take any res tomorrow and
see if it clears up, but I would like to know if res does have a cumulative effect
anyway.
My dad is still using vicadan in the mornings...but feels he is getting positive
results from Res the rest of the day. He refuses to try the DMSO. The label
warning scared him.
Albert, how long does it take for your hives to clear up after you stop res,
and/or if you continue to take the Res, do they still clear up?

[maxwatt]

I have no idea if this is from resveratrol or something else,
but I broke out in massive hives on my right thigh this evening. They don't itch but
they look terrible. They covered the whole front and outside of my thigh.
I haven't introduced any new supplement, and I didn't change the amount of res, so
I am wondering can the reaction be from a cumulative effect of res?maybe dmso?
And why my thigh? I've never applied it there. I won't take any res tomorrow and
see if it clears up, but I would like to know if res does have a cumulative effect
anyway.
My dad is still using vicadan in the mornings...but feels he is getting positive
results from Res the rest of the day. He refuses to try the DMSO. The label
warning scared him.
Albert, how long does it take for your hives to clear up after you stop res,
and/or if you continue to take the Res, do they still clear up?

Does an anti-histamine such as claritan relieve the hives?
I forget which foot has the neuroma you use DMSO and resveratrol on, right or left. If the hives, neuroma and DMSO are on the same side, maybe some contamination where you apply the DMSO is causing this. I have heard of clothing dyes (as used in socks and shoes) that can cause allergic reaction like this, particularly when combined with irritation as from tight clothes. I once had a red shirt that gave me hives only if I sweated when I wore it.

[missminni]

Does an anti-histamine such as claritan relieve the hives?
I forget which foot has the neuroma you use DMSO and resveratrol on, right or left. If the hives, neuroma and DMSO are on the same side, maybe some contamination where you apply the DMSO is causing this. I have heard of clothing dyes (as used in socks and shoes) that can cause allergic reaction like this, particularly when combined with irritation as from tight clothes. I once had a red shirt that gave me hives only if I sweated when I wore it.

The neuroma is on both feet, and I applied the DMSO on both feet. The hives don't itch, so I don't really need an anti-histamine.
I didn't wear any tight clothes yesterday or anything that I never wore before.
The only think new I added to my regimen was that Avon face cream with that ingredient
2-AMINO-4,5-DIMETHYLTHIAZOLE HBR
among many other ingredients, but I didn't put it on my thigh. just my face. Maybe something in the cream??
It is a very active cream. I like it. It doesn't make my face break out, but I did read where it made other people
break out.
Still, regardless of the hives, I would like to know if Res does have a cumulative effect. Can one expect to get
better the longer they use it, or will the improvement they first notice be as good as it gets?

[stephen_b]

I just mixed in 2g of 99% transresveratrol in about 8 ounces of water and a tablespoon of soy lecithin. The resulting mix was very clumpy, with much of the resveratrol still visible.

On the other hand, when I mix the same amount of resveratrol into a tablespoon (30ml) of cod liver oil, it goes completely into solution and the resulting mix is clear.

Maybe cod liver oil is a better medium to use for oral resveratrol delivery...

Stephen

[missminni]

I just mixed in 2g of 99% transresveratrol in about 8 ounces of water and a tablespoon of soy lecithin. The resulting mix was very clumpy, with much of the resveratrol still visible.

On the other hand, when I mix the same amount of resveratrol into a tablespoon (30ml) of cod liver oil, it goes completely into solution and the resulting mix is clear.

Maybe cod liver oil is a better medium to use for oral resveratrol delivery...

Stephen

I'm sure it is, if you can stand the taste of cod liver oil. Milk is great too. it dissolves with no effort in milk.
but the best of all is Jack Daniels. I highly recommend it for your evening dose. It not only dissolves in
the blink of an eye, it actually tastes pretty good too. (if you like JD)

[maxwatt]

I just mixed in 2g of 99% transresveratrol in about 8 ounces of water and a tablespoon of soy lecithin. The resulting mix was very clumpy, with much of the resveratrol still visible.

On the other hand, when I mix the same amount of resveratrol into a tablespoon (30ml) of cod liver oil, it goes completely into solution and the resulting mix is clear.

Maybe cod liver oil is a better medium to use for oral resveratrol delivery...

Stephen

I use a tea-ball to mix the lecithin into the water; not one with little holes, but a wire-mesh that allows quick mixing. Passing the mixture through a fine mesh strainer works too, if you don't have a tea-ball. This results in a translucent whit-colored liquid, to which I stir in the finest powdered resveratrol I can get. Not all the resveratrol particles are sufficiently fine to be bound by the lecithin. Some powder settles to the bottom, I stir that up and drink it too.

Yes, it dissolves completely in oils, and this is a good way to take it. No comparative studies have been done for resveratrol, but for CoQ10, similarly not water soluble, they have found that highest blood levels are obtained with a dispersant like lecithin (in this case they used cyclodextrin), followed closely by oil-dissolved CoQ10. Both methods were much better than capsules of dry powder.

It is possible that use of dispersant like lecithin results in better delivery at the cellular membrane. We do not really know which is better, but all methods of delivery seem to have worked for at least some people.

I also like to take 500 mg of 98% in the evening, sublingually: I place the powder under by tongue, and sip some Jack Daniels (or Vodka) 80 proof, diluted with water 1 to 1 ratio. I let it moisten the powder under my tongue, which seems to dissolve, and be absorbed by the mucosal membrane on the floor of the mouth. I use only 30 ml of spirits, which is one ounce. Not enough to get drunk, which is not the point.

[edward]

I had been putting off reading this monster thread as I have been very busy with school. Anyways I got through this entire thread last night.... Very interesting


This morning I tried the Resveratrol 99% (megaresveratrol brand) EtOH micronization, followed by the PEG (miralax) for the first time. Using roughly 500-700mg of Res and Vodka (1.5 oz of vodka) as I couldn't find Everclear. The Res did not get fully dissolved in the Vodka (after about 3 min of stirring) and the solution remained somewhat milky but I assume that some did. When I added the Miralax water to the Vodka Res solution the end product became even more milky thus leading me to believe that the Res that was dissolved was coming out of solution. The end result resembled Lucid's original pictures after stirring for a few minutes. After consuming my quercetin, grape seed, milk thistle, bioperine, curcumin, green tea, vitamin c, fish oil etc. etc. I chugged the Res Vodka Miralax Water mixture (yuck). Have noticed a stimulant effect (above and beyond what I get from my normal morning nutrient/vitamin/drug coctail (my biomarker for increased resveratrol activity as I am very sensitive to the stimulant effects of anything, in particular the initial 2 weeks of Res stimulation).

I am not sure I want to perform this experiment every morning as my mornings are rushed as it is, and I am still not so sure about taking roughly a shot of Vodka each morning.

One note on the DMSO idea. I mentioned DMSO as a vehicle last summer and created a thread but it didn't catch on, Ive tried it a few times and can say that subjectively it definitely yields a degree of stimulation above and beyond my normal oral dosage (again my biomarker for initial increased Res concentration in the blood). I stopped the DMSO delivery simply because its not very convenient, I am hoping Anthony or someone gets some data on keeping Res dissolved in a DMSO solution (stability and all) for easy application, as well as some actual blood levels for various delivery systems. If I am going to spend this much time each morning micronizing and mixing Res, applying with DMSO, maybe doing the sublingual thing I'd like to know for sure what I am doing is actually having an effect on blood levels

edit: An additional thought on the Miralax PEG issue. PEG is not absorbed by the intestines (apparently thats how it works as a laxitive it stays in the intestines and sucks water in and then passes out in ones stool), so my fear is that by binding to or encapsulating the Res we may be inadvertently preventing some of the Res from being absorbed... Which leads me to think that lecithin might be better since it is actually absorbed. If anyone has any information or a different perspective on this let me know.

Edited by edward, 16 December 2007 - 12:35 AM.

[stephen_b]

missminni, the cod liver oil has orange peel oil in it, tastes great. Unflavored oil, I agree, is awful.

I tried another experiment a little while ago. I wonder if a high alcohol content is needed when taking resveratrol orally. I found that 500 mg resveratrol dissolved quite nicely in a third of a glass of wine. You could see some resveratrol in suspension, but nothing settled out after 5 minutes.

Stephen

[missminni]

missminni, the cod liver oil has orange peel oil in it, tastes great. Unflavored oil, I agree, is awful.

I tried another experiment a little while ago. I wonder if a high alcohol content is needed when taking resveratrol orally. I found that 500 mg resveratrol dissolved quite nicely in a third of a glass of wine. You could see some resveratrol in suspension, but nothing settled out after 5 minutes.

Stephen

what brand of cod live oil do you use...I'd like to try it.
I actually mix my Jack with water 50/50.
It's too strong otherwise.
I guess the most appropriate way to drink it would be in a glass of red wine.

[stephen_b]

what brand of cod live oil do you use...I'd like to try it.

I use Nordic Naturals (you can get it at iHerb.

I guess the most appropriate way to drink it would be in a glass of red wine.

You're talking to a card-carrying member of the ABC club. (Anything but chardonnay.)



Stephen

[missminni]

what brand of cod live oil do you use...I'd like to try it.

I use Nordic Naturals (you can get it at iHerb.

I guess the most appropriate way to drink it would be in a glass of red wine.

You're talking to a card-carrying member of the ABC club. (Anything but chardonnay.)



Stephen

LOL
me too.
thanks for the link

[krillin]

I've been playing around with the proportion of Miralax to resveratrol recently. I've increased the dose of Miralax up to about 3 grams, based on my observation that I can get a small quantity of resveratrol to appear to dissolve in an aqueous solution of Miralax. My interpretation is that I'm forming a soluble complex between the two compounds, but with my earlier dose of about a gram of Miralax, this complex-forming ability was quickly saturated. The ability of the PEG to solubilize resveratrol appears to be roughly linear in PEG concentration, on the order of roughly 50-100mg resveratrol per gram of Miralax. At one gram I had no problem with laxative effects. At three grams every other day, I'm noticing looser stools, but nothing that's a problem. Miralax is a pretty gentle laxative. All it does is cause the stool to hold more water. You'd probably get a similar effect if you just drank a lot more liquid. (Which is a pretty good laxative itself, imho).

How much water do you use per gram of Miralax?

[niner]

I've been playing around with the proportion of Miralax to resveratrol recently. I've increased the dose of Miralax up to about 3 grams, based on my observation that I can get a small quantity of resveratrol to appear to dissolve in an aqueous solution of Miralax. My interpretation is that I'm forming a soluble complex between the two compounds, but with my earlier dose of about a gram of Miralax, this complex-forming ability was quickly saturated. The ability of the PEG to solubilize resveratrol appears to be roughly linear in PEG concentration, on the order of roughly 50-100mg resveratrol per gram of Miralax. At one gram I had no problem with laxative effects. At three grams every other day, I'm noticing looser stools, but nothing that's a problem. Miralax is a pretty gentle laxative. All it does is cause the stool to hold more water. You'd probably get a similar effect if you just drank a lot more liquid. (Which is a pretty good laxative itself, imho).

How much water do you use per gram of Miralax?

I've used about 135ml regardless of the amount of Miralax. No science behind it, it just seemed like a reasonable amount. I've always added about an equal amount of fruit juice before drinking it to mask the ungood taste of the brew.

[niner]

An additional thought on the Miralax PEG issue. PEG is not absorbed by the intestines (apparently thats how it works as a laxitive it stays in the intestines and sucks water in and then passes out in ones stool), so my fear is that by binding to or encapsulating the Res we may be inadvertently preventing some of the Res from being absorbed... Which leads me to think that lecithin might be better since it is actually absorbed. If anyone has any information or a different perspective on this let me know.

Interesting idea, Edward. You may have something there. For the time being I'll hope that the PEG is transporting the resveratrol to the lumenal surface of the gut where it equilibrates into the membrane. This convenient fiction is not entirely improbable, as resveratrol, with a logP of a bit over 3, is about a thousand times more likely to be found in a membrane-like (as modeled by n-octanol) environment that in the surrounding water. PEG is a linear polyether, so it isn't going to encapsulate the resveratrol in the way that a cyclodextrin would. I should try to pick up some lecithin, although it will be more of a hassle to dissolve. I don't suppose anyone knows of a convenient source for Tween, or something that approximates it? I'm pretty much ruling out cyclodextrins on the basis of cost.

[sUper GeNius]

An additional thought on the Miralax PEG issue. PEG is not absorbed by the intestines (apparently thats how it works as a laxitive it stays in the intestines and sucks water in and then passes out in ones stool), so my fear is that by binding to or encapsulating the Res we may be inadvertently preventing some of the Res from being absorbed... Which leads me to think that lecithin might be better since it is actually absorbed. If anyone has any information or a different perspective on this let me know.

Interesting idea, Edward. You may have something there. For the time being I'll hope that the PEG is transporting the resveratrol to the lumenal surface of the gut where it equilibrates into the membrane. This convenient fiction is not entirely improbable, as resveratrol, with a logP of a bit over 3, is about a thousand times more likely to be found in a membrane-like (as modeled by n-octanol) environment that in the surrounding water. PEG is a linear polyether, so it isn't going to encapsulate the resveratrol in the way that a cyclodextrin would. I should try to pick up some lecithin, although it will be more of a hassle to dissolve. I don't suppose anyone knows of a convenient source for Tween, or something that approximates it? I'm pretty much ruling out cyclodextrins on the basis of cost.

Polysorbate 80 is readily available, and it's cheap (do a google search,) but I wonder about purity. Tastes pretty bad too.

[ilanso]

I've just found my most convenient way to mix it in the morning: 3g into 15-25ml flaxseed oil. It beats fish oil taste-wise (IMO) and it very quickly converges to a white cream a la ranch dressing. An added perversion would be to spread it on a slice of bread/toast/Wasa. It reminded me of the way they eat olive oil on bread in Spain (minus the sun-dried tomatoes). On second thought, adding some garlic or other spices may turn this into the world's most healthful dressing (competing with spreads such as Benecol). Is a gourmet thread in order?
I also think walnut/olive oil could be substituted. For those of us not fans of carbs, tofu may make a good substrate. Or even a Julienne. Don't make it too spicy or the dog wouldn't like it
The idea here is to turm the yuck factor into yum whenever possible.

[edward]

Here is a link for reagent grade (pretty darn good) Tween-80 (aka polysorbate 80). Heavy metals (such as Pb): <0.001%

Its pretty cheap to $28.70 per liter (33ish ounces)

http://www.bio-world...ItemCode=730225

Personally I am going to go for it. Much better than PEG I would think and at least Tween was the actual surfactant/emulsifier used in Sirtis' 501 preparation. Maybe not as convient as your local drug store but not bad. Just one more thing to order... time to break out the credit card... again...

[missminni]

I've just found my most convenient way to mix it in the morning: 3g into 15-25ml flaxseed oil. It beats fish oil taste-wise (IMO) and it very quickly converges to a white cream a la ranch dressing. An added perversion would be to spread it on a slice of bread/toast/Wasa. It reminded me of the way they eat olive oil on bread in Spain (minus the sun-dried tomatoes). On second thought, adding some garlic or other spices may turn this into the world's most healthful dressing (competing with spreads such as Benecol). Is a gourmet thread in order?
I also think walnut/olive oil could be substituted. For those of us not fans of carbs, tofu may make a good substrate. Or even a Julienne. Don't make it too spicy or the dog wouldn't like it
The idea here is to turm the yuck factor into yum whenever possible.

Great ideas. BTW, the dogs looove spicy. What about a curry dressing?
Do you do 3g just once a day. Or do you do more again later?
I have been doing 400 mg 3x a day (the middle time being with DMSO) and i am wondering if I did a larger
dose all at once if I would get more benefit. I've been doing the 100mg/10lb ratio...but I see most
are doing much more.
What do you think? What effect do you find from the larger dose?

[edward]

Placed an order with the Bioworld people for Tween-80, but received an error message after submitting my credit card, tried it again and same thing. I will call them tomorrow or Monday and see what they say.

[ilanso]

QUOTE (ilanso @ 16-Dec 2007, 01:38 AM) *
I've just found my most convenient way to mix it in the morning: 3g into 15-25ml flaxseed oil. It beats fish oil taste-wise (IMO) and it very quickly converges to a white cream a la ranch dressing. An added perversion would be to spread it on a slice of bread/toast/Wasa. It reminded me of the way they eat olive oil on bread in Spain (minus the sun-dried tomatoes). On second thought, adding some garlic or other spices may turn this into the world's most healthful dressing (competing with spreads such as Benecol). Is a gourmet thread in order? shifty.jpg
I also think walnut/olive oil could be substituted. For those of us not fans of carbs, tofu may make a good substrate. Or even a Julienne. Don't make it too spicy or the dog wouldn't like it wink.gif
The idea here is to turm the yuck factor into yum whenever possible.

Great ideas. BTW, the dogs looove spicy. What about a curry dressing?
Do you do 3g just once a day. Or do you do more again later?
I have been doing 400 mg 3x a day (the middle time being with DMSO) and i am wondering if I did a larger
dose all at once if I would get more benefit. I've been doing the 100mg/10lb ratio...but I see most are doing much more.
What do you think? What effect do you find from the larger dose?

I do another 3g at night. That mg/kg ratio is more of a fiction until we have affordable ways to personally measure the tissue bioavailability - or someone like Anthony publishes such results at different intake levels. Until then, we only have a few studies and Albert (and I am no longer sure about him either) as a resvemeter.
Like I said upthread, the ultimate effect I expect to see is immortality, which isn't one of the easiest things to detect (like "so far so good"). However, my dog, who is 7, might lead the way, although she is not getting the same dose and we are back to animal vs human testing. And it will still take at least 8 good years of her living until I can be surer.
I am thinking adopting very old dogs could be a way to kill two birds with one stone (cruel pun not intended). Didn't they just open some ASPCA's Pet Adoption Centers in Manhattan recently with the idea of New York being a 0-euthanasia city? Put on Resv, they live longer, we get the feedback.
That said, in addition to the cure for my (our) congenital disease, I wouldn't mind detecting a few signs of increased well-being or stamina ("The incredible lightness of being") along the way.

[ilanso]

Here is a link for reagent grade (pretty darn good) Tween-80 (aka polysorbate 80). Heavy metals (such as Pb): <0.001%

Its pretty cheap to $28.70 per liter (33ish ounces)

How about: http://www.vitaminwo...p...ID=&CPID=21

Polysorbate 80, 8 fl. oz. Liquid $6.99

Maybe not as pure, but fit for human consumption.

[edward]

Here is a link for reagent grade (pretty darn good) Tween-80 (aka polysorbate 80). Heavy metals (such as Pb): <0.001%

Its pretty cheap to $28.70 per liter (33ish ounces)

How about: http://www.vitaminwo...p...ID=&CPID=21

Polysorbate 80, 8 fl. oz. Liquid $6.99

Maybe not as pure, but fit for human consumption.

I saw that but $28.70 for 1 liter is just as good of a deal. Furthermore the Polysorbate at vitamin world and other sites is marketed for cosmetic purposes as in mix it with creams for topical application. Personally I wouldn't drink it. If I am going to use raw chemicals for something to consume Ill go for lab quality, preferably reagent grade.

edit: that product in particular has biotin and niacin in it and is marketed to be applied to the scalp

Edited by edward, 16 December 2007 - 08:19 AM.

[sUper GeNius]

Can t-res be smoked?

[maxwatt]

Can t-res be smoked?

No, but it could be snorted but it might burn.

Polysorbate 80 is said to taste like vomit. I've not tried it.

One still needs micronized resveratrol for use with a surfactant; the powder I use has many very small particles, so some of it is effectively micronized, but the larger particles settle to the bottom of a PEG or a lecithin mixture. Lecithin, if you strain out the lumps that don't dissolve, is usable.

Dissolved in oil is probably very nearly as good as using a surfactant like PEG, judging by studies with other supplements that are not water-soluble. Garlic oil is an interesting possibility, for it contains DMSO and has a similar ability to act as a carrier. It would have to be diluted with other oils not to burn your mouth, and it could be applied topically. It raises the possibility of transdermal application, both orally and topically, with your salad oil. We can make the world's healthiest salads. I can market Garlic-Ranch Dressing with Resveratrol!

For dosage, no one knows the optimum. There does seem to be a linear dose-response relationship, and it has vary low toxicity.

For frequency, there are two theories: peak serum level or highest continuous serum level (maximum area under the curve). Some hints in the Sirtris presentations have led me to believe they are trying for the latter. Also, there is a secondary peak in serum levels about six hours after taking it, due to hepatic recycling: the liver dumps the glucoronated resveratrol into your colon, where bacteria de-glucoronate it, and it gets absorbed back into your blood. I take two daily doses of res, about 12 hours apart. In the morning two to three grams with lecitin, sometimes in milk. I the evening, 500 mg sublingually with a beverage that is diluted to 20% alcohol, or transdermally with DMSO. I believe the sublingual and transdermal methods result in higher peak serum levels, but that there are benefits to administration in the gastro0intestinal track as well.

Edited by maxwatt, 16 December 2007 - 11:25 AM.

[stephen_b]

I also like to take 500 mg of 98% in the evening, sublingually: I place the powder under by tongue, and sip some Jack Daniels (or Vodka) 80 proof, diluted with water 1 to 1 ratio.

I tried this last night with a bit of vodka (far less than a shot) and it seemed to work very well. Maxwatt, it's may be the case that you get more resveratrol this way than via your oral method. Looking forward to any upcoming plasma level testing with this and transdermal DMSO.

Stephen

[missminni]

I do another 3g at night. That mg/kg ratio is more of a fiction until we have affordable ways to personally measure the tissue bioavailability - or someone like Anthony publishes such results at different intake levels. Until then, we only have a few studies and Albert (and I am no longer sure about him either) as a resvemeter.
Like I said upthread, the ultimate effect I expect to see is immortality, which isn't one of the easiest things to detect (like "so far so good"). However, my dog, who is 7, might lead the way, although she is not getting the same dose and we are back to animal vs human testing. And it will still take at least 8 good years of her living until I can be surer.
I am thinking adopting very old dogs could be a way to kill two birds with one stone (cruel pun not intended). Didn't they just open some ASPCA's Pet Adoption Centers in Manhattan recently with the idea of New York being a 0-euthanasia city? Put on Resv, they live longer, we get the feedback.
That said, in addition to the cure for my (our) congenital disease, I wouldn't mind detecting a few signs of increased well-being or stamina("The incredible lightness of being") along the way.

lol
how long doing 6 g?
what about physical appearance...skin, hair....notice any improvement...

[luminous]

I take two daily doses of res, about 12 hours apart. In the morning two to three grams with lecitin, sometimes in milk. I the evening, 500 mg sublingually with a beverage that is diluted to 20% alcohol, or transdermally with DMSO. I believe the sublingual and transdermal methods result in higher peak serum levels, but that there are benefits to administration in the gastro0intestinal track as well.

maxwatt-

Could you please answer a few questions about your regimen...or perhaps direct me to a previous post with all the details?
1, Do you dissolve the lecithin in the milk, or do you take it in capsule/tablet form?
2. Do you dissolve the resveratrol in the milk, or do you take it in capsule/tablet form?
3. What is in the sublingual beverage besides 1/5 alcohol?
4. Are you using grain alcohol or something else?
5. Where do you get your DMSO, and what brand/type do you use?
6. Finally, what brand of resveratrol do you use, and what concentration (ie, 50%, 98%)?

All of this may have been answered, so forgive me for asking again if that's the case. I have limited free time and can't read each and every post. I'm asking you these things because I've gathered that you have a true understanding of the obstacles faced with getting resveratrol to "stick".

My understanding, on the other hand, is limited. If I'm interpreting correctly, the problem appears to be that (putting it simply) while resveratrol is readily absorbed into the bloodstream, the substance seems to rapidly deteriorate after that. Please correct me if I'm wrong. Thanks!

http://dmd.aspetjour...ract/32/12/1377

[maxwatt]

I take two daily doses of res, about 12 hours apart. In the morning two to three grams with lecitin, sometimes in milk. I the evening, 500 mg sublingually with a beverage that is diluted to 20% alcohol, or transdermally with DMSO. I believe the sublingual and transdermal methods result in higher peak serum levels, but that there are benefits to administration in the gastro0intestinal track as well.

maxwatt-

Could you please answer a few questions about your regimen...or perhaps direct me to a previous post with all the details?
1, Do you dissolve the lecithin in the milk, or do you take it in capsule/tablet form?
I use 98%+ powder that I import and test at a Chicago lab. I dissolve lecithin in water, strain the undissolved lumps -- they feel like snot to me-- then mix in the resveratrol; with milk, I just stir the res into the milk. It binds to the milk proteins and gets released during digestion. It's pain-relieving effects are undiminished, so I believe it is still bio-available with milk.
2. Do you dissolve the resveratrol in the milk, or do you take it in capsule/tablet form?
3. What is in the sublingual beverage besides 1/5 alcohol? I use 80 proof beverage, cut with an equal amount of water, so yes, that's 20% ethanol or exactly 1/5. I have used plain water, or pomegranate juice and it seems to be absorbed this way, but not as well. I once posted a link to a paper on sublingual and buccal absorbtion; the mucosa under the tongue and in the cheeks by the gums does absorb a certain amount of hydrophobic substances; any liquid helps disperse it sufficiently.
4. Are you using grain alcohol or something else?My brother uses beer, some have used wine. Why not champaign?
5. Where do you get your DMSO, and what brand/type do you use?Look HERE Use the 70% DMSO 30% water or it will sting.
6. Finally, what brand of resveratrol do you use, and what concentration (ie, 50%, 98%)?I use 98% extract. I buy by the kilo, as I am supplying my extended family and several dogs. By the way the 9 year-old terrier seems to have regenerated to age 3; Her arthritis is gone judging by the way she moves. PM or email me if you want source information.

All of this may have been answered, so forgive me for asking again if that's the case. I have limited free time and can't read each and every post. I'm asking you these things because I've gathered that you have a true understanding of the obstacles faced with getting resveratrol to "stick".

My understanding, on the other hand, is limited. If I'm interpreting correctly, the problem appears to be that (putting it simply) while resveratrol is readily absorbed into the bloodstream, the substance seems to rapidly deteriorate after that. Please correct me if I'm wrong. Thanks!

http://dmd.aspetjour...ract/32/12/1377

Short answer: I believe enough gets through metabolic processing to have an effect. If resveratrol weren't sulfonated, we would need much less of it. Sirtris' research seems geared to dealing with this. I believe the use of a surfactant, be it lecithin, PEG or Polysorbate/Tween80, partly overcomes the sulfonation problem. Taking it sublingually or transermally with DMSO bypasses first-pass metabolization by the liver, which is where blood first goes from your digestive tract. So this results in higher initial blood levels, and hopefully enough is absorbed by your cells to activate SirT1, et al. before it gets to the liver and gets sulfonated.

An interesting alternative I'm exploring is Luteolin. Like resveratrol, it activates the sirtuin genes, about 50% as effectively in the in vitro assays. But unlike resveratrol, the glucoronates seem to be converted back to the active substance more easily, so higher cellular levels can in theory be obtained. (The refereces are not the best, this needs confirmation.) Preliminary impression from self-testing: it is as effective, and at smaller doses, for pain relief and for anti-inflammatory action. At least one manufacturer is using it in a formulation with resveratrol to inhibit sulfonation and glucoridation of resveratrol. It gets conjugated,, and spares the resveratrol. at least that's the rational. I've an inexpensive source for luteolin, am determining if I should buy it in quantity, and mix it with my resveratrol. I do know that if the FDA acts to ban resveratrol, I will be using luteolin instead.

Edited by maxwatt, 16 December 2007 - 05:35 PM.