"500 club" 500mg of trans-resveratrol per day

Someone did offer t-resv. transdermal patches for sale, some time ago, but the shipment has apparently been delayed....indefinitely?

Hmm. About the transdermal patch I don't know. I'd get a sample before
buying more, because TRES is a known irritant of skin and mucous membranes.
I handled some 99% pure TRES mixing it with a lecithin/oil emulsion to increase
bioavailability... with just one glove on, I noticed itching/stinging sensations
on the skin. Not bad, but I didn't have long contact with a lot of it. Perhaps
it varies from person to person, but at least for many, concentrated TRES
on the skin should be rather irritating...

In a different thread, I talked about taking resveratrol to help with my hip osteoarthritis. (I am moving the discussion of my experiences to this thread because it seems to be the more appropriate area.) The experiment has started. On an empty stomach, I took 1 gram of resveratrol, mixed in a lecithin/water mixture. The taste was fairly bland, much less bad than I had been expecting. I also took 100 mg LEF resveratrol with quercetin at the same time (I have about 10 capsules left and want to finish them slowly as to not take to much quercetin on any one day.

I am planning to take this mixture for a week and then reevaluate. I will let everyone know of my progress.

Albert

Is there anyway we can take a poll?
Approx how many RSV users?
Plus; 500, 750 and 1,000 MG/day range.
We might have enough users to contribute valuable research data.

I found this chart at what I assume is Bill Sardi's Resveratrol site. He has put together a collection of most of the world's maladies and references studies that show how resveratrol affects them. Everything from prolonging life to prolonging erections. Check it out. http://www.resveratr....com/page70.htm

THE MANY BIOLOGICAL ACTIONS OF RESVERATROL

A work in progress

Biological action
Reference

Preserves or stimulates superoxide dismutase (antioxidant)
J Agriculture Food Chemistry 2005 May 18; 53(10):4182-6; Free Radical Biology Medicine 2003 Apr 1; 34(7):810-7

Elevates glutathione (antioxidant)


J Agric Food Chem. 2005 May 18; 53 (10):4182-6; Archives Biochemistry Biophysics 2000 Sep 15; 381 (2):253-63

Elevates catalase activity (antioxidant)
Life Science 2003 May 2; 72 (24):2741-50

Prolongs life of cells via Sirtuin 1 gene


Trends Pharmacological Sciences 2005; 26:94-103

Anti-histamine action (allergy)


Planta Medica 2004 Apr;70(4):305-9,



Anti-inflammatory agent


J Chemotherapy 2004 Nov;16 SuppI 4:3-6;

J Environ Pathology Toxicology Oncology 2004; 23(3):215-26

COX-2 inhibitor (anti-inflammatory)


Inflammation Research 2005 Apr; 54(4):158-62

Potentially helpful for rheumatoid arthritis
International Immunopharmacology 2005 May;5(5):849-56

Inhibits pancreatitis


World J Gastroenterology 11: 3171-74,2005

Promotes DNA repair (via Sirtuin 1 gene)


Nature 2003 Sep 11; 425 (6954):191-6

Inhibits abnormal new blood vessel formation (angiogenesis)
J Physiology Pharmacology 2005 Mar;56 Supp11:51-69

Inhibits dietary sugar absorption


Journal Natural Products 2001; 64:381-84



Regulates blood sugar


J Agric Food Chemistry 2005 May 4; 53(9):3403-7

Normalizes blood pressure


J Hypertension 2000 Dec; 18(12): 1833-40



ACE Inhibitor properties


European J Pharmacology 2005 May 16; 515(1-3): 1-9

Prevents blood clots


Blood Coagulation Fibrinolysis 2004 Sep;15(6):441-6

Reduces LDL cholesterol


Life Sciences 2003 Aug 1; 73(11):1393-400



Reduces triglycerides


Life Sciences 2003 Aug 1; 73(11): 1393-400



Raises HDL "good" cholesterol


J Agric Food Chemistry 2005 May 4; 53(9):3403-7



Biological action
Reference

Inhibits arterial plaque w/o altering

cholesterol
IntJMol Med. 2005 Oct;16(4):533-40.



Calms effects of estrogen (estrogen

blocker)
J Steroid Biochemical Mol Biology 2005 Apr;94(5):431-43

Prevents bone loss


J Medicinal Food 2005 Spring;8(1):14-9



Protects retinal cells (retinal pigment

epithelium)


Chemical Biological Interaction 2005 Jan 15; 151(2): 143-9



Increases sperm count
J Nutrition 2005 Apr; 135(4) :757-60

Inhibits viral growth (influenza)


J Infectious Disease 2005 May 15; 191 (10): 1719-29

Inhibits viral growth (HIV)
J Pharm Science 2004 Oct; 93 (10):2448-57

Inhibits viral growth (herpes)


Antiviral Research 2004 Jan;61(1);19-26; 1999 Oct;43(3): 145-55

Antibiotic against bacteria (Chlamydia)


Atherosclerosis 2003 Dec;171(2):379-80



Antibiotic against bacteria (H. pylori)


Am J Gastroenterology 2003 Jun;98(6):1440-1



Inhibits growth of fungi (yeast, mold)


J Agriculture Food Chemistry 2003 Feb 26:51 (5): 1464-8

Inhibits initiation of tumors


Proc NatI Sci Counc Repub China B. 1999 Jul;23(3):99-

106



Inhibits growth of tumors 2


Drugs Experimental Clinical Research 1999:25(2-3):65-77

Inhibits spread of tumors 3
Proc NatI Sci Council Repub China B. 1999 Jul;23(3):99-106

Targets multiple genes (silencing/activation)
I J Nutritional Biochemistry. 2005Aug;16(8):449-66

Protects brain cells__________


Ann N Y Academy Sciences 2003 May;993:276-86

Inhibits hepatitis (liver inflammation)____


I Hepatogastroenterology 2002 Jul-Aug;49(46):1102-8

Eradicates plaque in brain (beta amyloid

toxicity)
British J Pharmacology 2004 Mar;141 (6):997-1005

Chelates metals (copper)_________


Biochem Pharmacology 1997 May 9; 53(9): 1347-55

Anti-leukemia agent__________


iLeukemia Lymphoma 2002 May;43(5):983-7


Inhibits prostate cancer


Cancer Epidemiology Biomarkers Prevention 2005 Mar;14(3):596-604

Inhibits breast cancer
Molecular Nutrition Food Research 2005 May;49(5):462-71

Inhibits melanoma
I Neoplasia2005Jan;7(1):37-47

Inhibits liver cancer
World J Gastroenterology 2003 Oct;9(10):2341-3

Inhibits brain cancer
[Molecular Cancer Therapy 2005 Apr;4(4):554-61

Inhibits pancreatic cancer
Pancreas 2002 Nov;25(4):e71-6



Inhibits kidney cancer
Cancer Biology Therapy 2004 Sep; 3(9):882-8

Inhibits colon cancer
International J Cancer. 2005 Jun 10; 15(2): 194-201

Inhibits ovarian and cervical cancer
Anticancer Res. 2004 Sep-ct;24(5A):2783-840.

Inhibits lymphoma
Cancer Letters 2001 Feb 10,163(1 ):43-9

Exhibits Viagra-like effects (nitric oxide)


Free Radical Biology Med. 2004 Mar 15;36(6):774-81

Novel molecule for organ transplants
World J Gastroenterology. 2005 Aug 14;11(30):4745-9



Non-toxic
J Nutrition 2002 Feb;132(2):257-60

I am 60 years old with blond/gray hair and I am using for abt 6 months 300 mg country life and noticed the last few months in my moustache, which was blond/gray, very dark hairs. Also noticed after cutting my hair on my head that it looked more dark. Are more ,older, people experience this effect?

I am 60 years old with blond/gray hair and I am using for abt 6 months 300 mg country life and noticed the last few months in my moustache, which was blond/gray, very dark hairs. Also noticed after cutting my hair on my head that it looked more dark. Are more ,older, people experience this effect?

You are the first to report hair-darkening effects here. In Chinese folk medicine, a medication from a plant that may have contained resveratrol, was believed to darken gray hair and restore youthful vigor.

IIRC, someone else previously reported hair darkening on at least one of the threads devoted to t-resv.

Ok, I am prolly going to catch a lot of flak for this but....

Have any of you noticed an improvement in the quality of your erections? (duck my head)

I'm 40 so approaching middle age. Like to think of myself as in-shape and following a good diet, largely vegetarian.

I'm not saying I had a problem before (no sir, not me) but since starting higher doses of t-res (also my daily green tea extract, Pom40P and occasional cocoa), I feel like I can get an erection whenever I want and drill thru wood w/ it. More like a teenager or young 20yr old in that regard. [thumb]

I"m sure it has to do w/ improved blood flow, increased NO, etc.


Ok, I"m ready for the flying fruit thrown my way.......

It depends on the partner....

just a guess, but hasn't resv been shown to sometimes show anti-e properties. With less conversion from test to estrogen, wouldn't that lead to more test activity and possibly increased libido?????

Yes, maxwatt, certainly a partner dependent phenomenon but I would think that would also affect frequency primarily. Riseboi, I am not talking libido per se although that may go hand in hand (is that a bon mot?) but quality of erections, eg, I think the proper phrase is tumescence.....

just a guess, but hasn't resv been shown to sometimes show anti-e properties.  With less conversion from test to estrogen, wouldn't that lead to more test activity and possibly increased libido?????

You are the first to report hair-darkening effects here. In Chinese folk medicine, a medication from a plant that may have contained resveratrol, was believed to darken gray hair and restore youthful vigor.

OK, I think I'm seeing darker hairs too. I've noticed them only in my beard, mainly it seems in areas that I normally shave. I might have seen some darkening on my head, but that's just as likely to be wishful thinking. But on my face and around my ears I really do seem to have some new thick dark hairs. This is all from 116 mg/day for several months. I've only recently gone up to ~350mg. So... Resveratrol or general hormonal wierdness? I don't know. It's a data point, or at least another anecdote.

although that may go hand in hand

My inner Beavis and Butthead are cracking up...

It depends on the partner....

Woody Allen said it: "Taking Resveratrol is like playing bridge: you either need a good partner or a better hand"

http://www.giantnutr...?products_id=66

Can anyone clarify how much trans-reservatrol is in a serving of this product? I'm not sure what the 50% means. :(

Can anyone clarify how much trans-reservatrol is in a serving of this product? I'm not sure what the 50% means. :(

The 50% reference is to the resveratrol content of the plant extract they are using as a resveratrol source. You can get both higher and lower purity extracts. In this case one serving (two pills) gives you 500 mg of the extract, so that's nominally 250 mg of resveratrol. A 50% extract will probably contain a significant amount of emodin, an intestinal irritant/laxative, so this may not be the best choice if you want to take a lot of it (250-1000 mg/day). Read some of the long resveratrol threads here (use the Search function) to learn all about it.

http://www.giantnutr...?products_id=66

Can anyone clarify how much trans-reservatrol is in a serving of this product?  I'm not sure what the 50% means. :(

Hi dannov, I read your post referring to 6-OXO Extreme; isn't Giant another one of Patrick Arnold's brands (ErgoPharm being the other)? If so, I'd think the resveratrol is from the same source as the resv in the 6-OXO E. you've been taking. I've also been taking 6-OXO E at 5 caps a day (1200mg of 50% resv). If taken in a single dose, I definitely notice a laxative effect from the emodin. If I take the 5 caps in two doses, I don't notice the effect.

The price looks to be competitive for 50% caps, but of course at a high daily dose a bottle wouldn't last long. Giant resv looks like it has the same ratio of quercetin to resveratrol as 6-OXO E, but significantly less piperine per dose (20mg in 6-OXO E vs. 6mg for an equivalent dose of Giant). I don't doubt that piperine increases absorption, but after reading a couple of piperine (trade name bioprene, I beleive) threads around here, I have my concerns about taking too much of the stuff. I may grab a bottle to dilute my 6-OXO E doses and decrease my piperine intake.

Aye, though I believe it's a different company, but the same person. I could be wrong.

I PM'ed Pat the other day on bodybuilding.com asking him why there was less Piperine, and he basically said that too much Pip was a cause of heartburn for himself (he uses about 2g a day of giant), so he lowered the amount. I'm assuming he figured it'd be the same for consumers as well.

From what I've read, Pip is simply a substance inherent in types of black pepper. However, everyone reacts differently to substances...hell, some are even immune to poison ivy. :D

And, don't even get me started on the laxative effect...1-2 times at work, and usually 2 times at home throughout the evening. One time 15 minutes apart at home...

Aye, though I believe it's a different company, but the same person.  I could be wrong.

....
And, don't even get me started on the laxative effect...1-2 times at work, and usually 2 times at home throughout the evening.  One time 15 minutes apart at home...

Search past posts on emodin; basically it's found in 50% extracts at levels between 3 and 5%, and is a potent laxative. It makes it impossible to ingest high dosages of resveratrol from 50% extracts. I think most of us on over 500 mg a day are using 98% or higher extracts/

That could also be where the Quercatin and Piperin come in though, Max.

Aye, though I believe it's a different company, but the same person.  I could be wrong.

....
And, don't even get me started on the laxative effect...1-2 times at work, and usually 2 times at home throughout the evening.  One time 15 minutes apart at home...

Search past posts on emodin; basically it's found in 50% extracts at levels between 3 and 5%, and is a potent laxative. It makes it impossible to ingest high dosages of resveratrol from 50% extracts. I think most of us on over 500 mg a day are using 98% or higher extracts/

I've heard people say that they seem to get a tolerance to the emodin (this was on bodybuilding.com - they actually though that it was the piperine in 6-OXO E). I had problems early on, even with split doses (600mg of 50% res twice a day), but adjusted pretty quickly. Now I'm taking 840mg of 50% res split into two doses and don't seem to have any emodin side effects. I do plan on upping that and will probably go to 98% - but not so much to eliminate emodin as to eliminate other potential impurities.

Proanthocyanidins as D2 agonists may explain some of the subjective effects of low purity extracts.

Pharmacol Biochem Behav. 2006 Oct;85(2):317-23.
Analysis of the antinociceptive effect of the proanthocyanidin-rich fraction obtained from Croton celtidifolius barks: evidence for a role of the dopaminergic system.
DalBó S, Jürgensen S, Horst H, Soethe DN, Santos AR, Pizzolatti MG, Ribeiro-do-Valle RM.
Departamento de Farmacologia, Centro de Ciências Biológicas, Bloco D, UFSC, Campus Universitário, Trindade, Florianópolis, SC, CEP 88040-900, Brazil.

In a previous study, we demonstrated the antinociceptive effect of 63SF, a proanthocyanidin-rich fraction obtained from Croton celtidifolius barks, in chemical and thermal behavioural models of pain in mice. The current study now investigate the possible mechanisms underlying the antinociceptive activity of 63SF in the formalin test, by using drugs which interfere with systems that are implicated in descending control of nociception. The antinociceptive effect of 63SF (11 mg/kg, i.p., given 30 min prior to 2.5% formalin) was not altered by pre-treatment of animals 45-50 min beforehand with either prazosin (alpha(1)-adrenergic antagonist; 0.15 mg/kg, i.p.), yohimbine (alpha(2)-adrenergic antagonist; 0.15 mg/kg, i.p.), ketanserin (5-HT(2A)-receptor antagonist; 1.0 mg/kg, i.p.), or l-arginine (substrate for NO synthase, 600 mg/kg, i.p.). On the other hand, treatment with sulpiride, an antagonist of dopaminergic D(2)-receptors (1.0 mg/kg, i.p., 45 min of pre-treatment), reversed the antinociceptive activity of 63SF. Pre-treatment of animals with reserpine (5 mg/kg, i.p., 24 h beforehand) did not alter the antinociceptive effect of 63SF. The current results support the view that the 63SF exerts antinociceptive effects by enhancing the activity of descending control, possibly by direct stimulation of dopaminergic D(2) receptors.

PMID: 17030059

I just had blood drawn, and I promise to post the results here, good or bad. I'm disappointed that so few others have shared their results in the past three or four months, including several people who said they planned to have blood work done by this summer. Results that are filtered by whether the subject is excited enough to report them are not very meaningful.

Meanwhile, here's an update on other objective measurements:

* Weight 76kg-78kg (depending on time of day).
* My VO2 max measurements these past few weeks have averaged around 70.
-- The lowest I've seen recently is 56 and I think one broke 80.
-- Average heart rate during the test is still often low, like 107, but occasionally as high as it used to be (120).
* Average blood pressure: 115/75 (sys. ranges from about 105 to 127).

I think the improvements have all been stable these past three months; you can search for my past posts if you really want to know.

Compare these to what I reported in my first post, after about six months at low dose:

My blood pressure has been all over the place, systolic ranging from 118 to 139 within an hour even when I've been sitting down, and I haven't noticed any change.  My VO2 max, as measured by an automated treadmill at the gym, has always been absurdly high, like in the 50's (averages 90th percentile -- if I were a teenager) since I started measuring it in 2004, and I think it's been a little higher recently, ranging from 56 to 71.

In late May. after my first post-resveratrol blood test, I raised my dose to 5 mg/kg/day, and for the past month or two I've been taking 7 or 8 mg/kg/day.

Very interesting, thanks for the info unglued!

I'm planning on getting my blood work next week and a physical in early October. My health plan only pays for one checkup a year and the last was done in Sept of 2006. I'll post results when they come in. BTW those VO2 readings seem astonishingly high. After all Lance Armstrong tested at 83.8 and he is a mutant. What testing machine did you use?

velopismo -- I've been measuring my VO2 score for three years with four identical-looking Star Trac treadmills (not sure of the model number), and the numbers were absurdly high from the beginning, as I said. Now they've improved; they used to be below the 90th percentile for a 20-year-old as often as they were above it. Unfortunately, I don't have access to any other types of machines for a second opinion -- the one time I did, the scores were significantly lower, but I was visiting a city at 1500m elevation and I live at sea level, so that's to be expected. It's not impossible that the numbers are accurate -- I'm no athlete, but let's put it this way: I don't use a car to reach the place where the treadmill is.

On the other hand, I suspect that after decades of walking I'm somehow more efficient at walking than the machine assumes I am. That is, my leg muscles may not be demanding as much oxygen for going 3.4mph (5.47 km/hr) as the formula assumes.

On the third hand (er, anyone else experience that symptom?), the mechanism by which resveratrol lets rats run better on treadmills is thought to be increasing the efficiency of their mitochondria, right? So I would actually hope the moderate improvement I think I've been seeing lately is not a true improvement in my VO2 max, strictly speaking -- i.e., not an improvement in my heart's ability to deliver oxygenated blood to my muscles. Instead, I would hope it's the resveratrol adding fresh mitochondria to my muscle cells, allowing them to do the same work with less oxygen and less oxidation.

I've already got the test results back. My HMO has computerized their system, and what used to take weeks to report now takes less than 12 hours. The blood is barely dry on the gauze.

To the extent that I'm looking for cholesterol improvements as a marker for resveratrol increasing my longevity, the results are disappointing. They're back to almost exactly the levels they were in my last pre-resveratrol blood work a year before (August 2006):

Total Cholesterol: 129
Triglyceride: 130
HDL: 40
LDL calculated = 129 - 40 - 130/5 = 63
Glucose, fasting: 84

See previous results here.

My results are consistent with two theories:
1. Although resveratrol may increase lifespan and even protect the cardiovascular system, it has no effect on cholesterol -- supported by two papers discussed before; see here and here.
2. Or, Longevinex's claim is right, and their fancy nitrogen packaging preserves so much more resveratrol activity (or the synergistic effects with their other ingredients is so significant) that it's more cost-effective than the cheaper brands. I used Longevinex during (northern) spring but then bought cheaper products (RevGenetics and Country Life and a little NSI) when I went to a higher dosage. However, as I said above, I've seen modest improvements in weight, VO2 max, and blood pressure that seem to be sticking.

Looking forward to more data from the rest of you.

I was under the impression that VO2max is the maximum rate at which you can use oxygen for energy production. It thus is influenced by the whole transport and use chain: lungs, heart, blood, and mitochondria.

http://www.healthsys...logy/vo2max.cfm

Increases in VO2 max are primarily the result of an increase in cardiac output and, to a lesser degree, to better extraction and utilization of oxygen by working muscles.

The additional mitochondria will increase your maximum oxidation rate. I'm not sure if fuel economy will be improved, but there will be less oxidation per mitochondrion during most of the day, so they should last longer.

..

Edited by david ellis, 10 September 2007 - 03:15 PM.