5 replies to this topic

[kitinje]

This is a very interesting publication:

from
Circulating endothelial progenitor cells: a new approach to anti-aging medicine?
Journal of Translational Medicine 2009, 7:106 doi:10.1186/1479-5876-7-106
Published: 15 December 2009 - pubmed
http://www.translati...content/7/1/106
full publication: http://www.translati...-5876-7-106.pdf

Endothelial dysfunction is associated with major causes of morbidity and mortality, as well as numerous age-related conditions. The possibility of preserving or even rejuvenating endothelial function offers a potent means of preventing/treating some of the most fearful aspects of aging such as loss of mental, cardiovascular, and sexual function. Endothelial precursor cells (EPC) provide a continual source of replenishment for damaged or senescent blood vessels. In this review we discuss the biological relevance of circulating EPC in a variety of pathologies in order to build the case that these cells act as an endogenous mechanism of regeneration. Factors controlling EPC mobilization, migration, and function, as well as therapeutic interventions based on mobilization of EPC will be reviewed. We conclude by discussing several clinically-relevant approaches to EPC mobilization and provide preliminary data on a food supplement, Stem-Kine, which enhanced EPC mobilization in human subjects.

The paper identifies possible interventions for maintaining levels of circulating endothelial cells and reversing age-associated degeneration. Of particular interest is new data demonstrating that Stem-Kine, a commercially available oral nutritional supplement, doubled the number of circulating endothelial progenitor cells in healthy volunteers after a 2-week period.

Couldn't find anything regarding stem-kine as a supplement except this TM registration as a Dietary and nutritional supplement:
http://www.trademark...e-77760973.html

Anyone knows something more about it?

Attached Files

•  epc.jpg   58.58KB   30 downloads

Edited by kitinje, 29 December 2009 - 09:55 PM.

[tunt01]

Interesting.

The paper describes the supplement as containing:

The nutritional supplement Stem-Kine (Aidan Products, Chandler, AZ)
contains: ellagic acid a polyphenol antioxidant found in numerous vegetables and fruits;
vitamin D3 which has been shown to mildly increase circulating progenitor cells; beta 1,3
glucan (previous studies have reported administration of various beta glucans to elicit
stem cell mobilization [150]), and a ferment of the bacterium, Lactobacillus fermentum.
Lactobacillus fermentum is generally regarded as safe, and has been in the food supply
for hundreds of years as a starter culture for the production of sour dough bread and
provides for its characteristic sour flavor. Extract of green tea, extract of goji berries, and
extract of the root of astragalus were added prior to the fermentation process. Green tea
extracts and some components of goji berries are known to mildly stimulate progenitor
cell release, and astragalosides and other molecules found in the root of astragalus are
known antioxidants that can prevent cellular damage secondary to oxidation.
Fermentation is known to increase the bioavailability of minerals, proteins, peptides,
antioxidants, flavanols and other organic molecules. Imm-Kine, another Lactobacillus
fermentum fermented product that includes beta 1,3, glucan has been safely distributed
for 9 years without reported side effects.

Stem-Kine is a trademarked product owned by http://www.aidanproducts.com/ - the product is listed here, but you cannot see the details.

it seems like an expanded version of Imm-Kine (which is just beta-glucan and probiotics).

you can basically recreate this by taking pomegranate juice (ellagic acid), goji berries, green tea, oatmeal (beta glucan source), vitamin d3, probiotics (yogurt?). plus astragulus.

Edited by prophets, 29 December 2009 - 11:56 PM.

[kitinje]

Interesting.
Stem-Kine is a trademarked product owned by http://www.aidanproducts.com/ - the product is listed here, but you cannot see the details.

it seems like an expanded version of Imm-Kine (which is just beta-glucan and probiotics).

you can basically recreate this by taking pomegranate juice (ellagic acid), goji berries, green tea, oatmeal (beta glucan source), vitamin d3, probiotics (yogurt?). plus astragulus.

Here is something more:
Studies have demonstrated a positive correlation
between the number of circulating stem cells and
health, wellness and regenerative capabilities.
Circulating stem cells are sourced from an
individual’s bone marrow. Through stimulating
the release of these circulating cells, Stem-Kine
may heighten regenerative activities. A critical
mechanism of Stem-Kine’s activity is protection of
the circulating stem cells from oxidative stress (See
Figure 2).
I see it contains 500% the DV of Vitamin-D3 (which seems to be related with mobilization)
You were right, it was not possible to see the details. But I tried again a couple of minutes ago and for some reason it now shows everything.
Full pdf, product details and study are attached.

Attached Files

•  epc.jpg   58.58KB   27 downloads
•  ox_stress.jpg   43.34KB   38 downloads
•  supp_contents.jpg   38.66KB   49 downloads
•  stem_kine_brochure.pdf   379.52KB   135 downloads

Edited by kitinje, 30 December 2009 - 11:59 AM.

[chrono]

They just did a follow-up study for Stem Kine (prophets posted the news story here).

Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects [PDF]
Journal of Translational Medicine 2010;8:34

The medical significance of circulating endothelial or hematopoietic progenitors is becoming increasing recognized. While therapeutic augmentation of circulating progenitor cells using G-CSF has resulted in promising preclinical and early clinical data for several degenerative conditions, this approach is limited by cost and inability to perform chronic administration. Stem-Kine is a food supplement that was previously reported to augment circulating EPC in a pilot study. Here we report a trial in 18 healthy volunteers administered Stem-Kine twice daily for a 2 week period. Significant increases in circulating CD133 and CD34 cells were observed at days 1, 2, 7, and 14 subsequent to initiation of administration, which correlated with increased hematopoietic progenitors as detected by the HALO assay. Augmentation of EPC numbers in circulation was detected by KDR-1/CD34 staining and colony forming assays. These data suggest Stem-Kine supplementation may be useful as a stimulator of reparative processes associated with mobilization of hematopoietic and endothelial progenitors.

Obviously not the best design, and some conflict of interest, but the results are still promising.

you can basically recreate this by taking pomegranate juice (ellagic acid), goji berries, green tea, oatmeal (beta glucan source), vitamin d3, probiotics (yogurt?). plus astragulus.

This is my first thought as well. For probiotics, I found a product called GI-48 containing Lactobacillus ferentum KLD, which is apparently one of the few strains able to make it through the GI tract successfully (9674129).

Edited by chrono, 25 April 2010 - 05:50 AM.

[niner]

They just did a follow-up study for Stem Kine (prophets posted the news story here).

Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects [PDF]
Journal of Translational Medicine 2010;8:34

The medical significance of circulating endothelial or hematopoietic progenitors is becoming increasing recognized. While therapeutic augmentation of circulating progenitor cells using G-CSF has resulted in promising preclinical and early clinical data for several degenerative conditions, this approach is limited by cost and inability to perform chronic administration. Stem-Kine is a food supplement that was previously reported to augment circulating EPC in a pilot study. Here we report a trial in 18 healthy volunteers administered Stem-Kine twice daily for a 2 week period. Significant increases in circulating CD133 and CD34 cells were observed at days 1, 2, 7, and 14 subsequent to initiation of administration, which correlated with increased hematopoietic progenitors as detected by the HALO assay. Augmentation of EPC numbers in circulation was detected by KDR-1/CD34 staining and colony forming assays. These data suggest Stem-Kine supplementation may be useful as a stimulator of reparative processes associated with mobilization of hematopoietic and endothelial progenitors.

Obviously not the best design, and some conflict of interest, but the results are still promising.

you can basically recreate this by taking pomegranate juice (ellagic acid), goji berries, green tea, oatmeal (beta glucan source), vitamin d3, probiotics (yogurt?). plus astragulus.

This is my first thought as well. For probiotics, I found a product called GI-48 containing Lactobacillus ferentum KLD, which is apparently one of the few strains able to make it through the GI tract successfully (9674129).

FWIW, they are not using live bacteria, but rather an extract of them. Hard to say what's in the extract; that would depend mightily on the extraction conditions, not to mention various possible pre-extraction steps, like what they feed the bacteria, and how they process them prior to extraction. This might be something like Epicor, where they feed all kinds of stuff to yeast, or maybe it's nothing and not that important. The details are all hiding behind the "proprietary blend", although they've applied for a patent, so it should be possible to find the patent application that might tell how it's done.

[chrono]

FWIW, they are not using live bacteria, but rather an extract of them. Hard to say what's in the extract; that would depend mightily on the extraction conditions, not to mention various possible pre-extraction steps, like what they feed the bacteria, and how they process them prior to extraction. This might be something like Epicor, where they feed all kinds of stuff to yeast, or maybe it's nothing and not that important. The details are all hiding behind the "proprietary blend", although they've applied for a patent, so it should be possible to find the patent application that might tell how it's done.

True, the bacteria were fermented. I misread my abstract because the strain is also named fermentum Didn't see it mentioned that they were extracted, though (unless you're referring to "food-derived").

I'm sure it would be difficult to reproduce their extract exactly, especially as the whole mix was fermented. But in approximating it with the ingredients, I assume that most of the same benefits would be derived, differing perhaps only in magnitude. I haven't read up on probiotics yet, but I don't picture substrains or fermentation process having that large an impact on the process of stem cell augmentation as a whole. In fact, I'm assuming that several of those ingredients are just there for general health. Could be wrong, though.

No luck finding the patent app with a quick search. I'll look more tomorrow (that is, unless another kind soul wants to help a patent-weary soul out...)

Edited by chrono, 25 April 2010 - 06:11 AM.