Recently I posted this summary of cryonics research history
http://www.imminst.o...post__p__408451
The current technical limits are that technology does not presently exist to preserve large organs with viability (ability to spontaneously recover function) at cryogenic temperatures. The largest organs so far recovered from cryopreservation are dog intestines
http://www.ncbi.nlm....?dopt=Ab stract
and a rabbit kidney
http://www.21cm.com/...hyORG5-3[1].pdf
The best cryonics can do is achieve morphological preservation of tissue by vitrifying, or attempting to vitrify it. See
http://www.alcor.org...rification.html
for a simple explanation of vitrification. See
http://www.alcor.org.../cambridge.html
for a longer explanation of the technical issues and current state of art.
Cryonics procedures are not static. Alcor did a major technology upgrade as recently as 2005.
http://www.alcor.org...technology.html
Current research foci are improving the quality of brain ultrastructure preservation, as documented by electron microscopy, as a prelude to the development of demonstrably reversible brain preservation with retention of viability (ability to spontaneously recover function) by contemporary criteria. There is also some research going on in documenting and improving cryoprotectant distribution through the whole body for organizations that perfuse the whole body (right now Alcor only). Alcor has also been working on a fully computer-controlled cryoprotectant perfusion system for whole bodies that would be comparable to the sophisticated research perfusion systems that have previously been used only for isolated organs.
The most revolutionary new technology expected to come on the cryonics scene within the new few years, being developed by Suspended Animation, Inc., based on published research by Critical Care Research, Inc. is fluorocarbon liquid ventilation for accelerated post cardiac arrest cooling
http://www.ncbi.nlm....pubmed/11719148